Label-free Quantitative Analysis of One-dimensional PAGE LC/MS/MS Proteome

Label-free quantitative analysis of one-dimensional PAGE LC/MS/MS proteome: application on angiotensin II-stimulated smooth muscle cells secretome.

A widely used method for protein identification couples prefractionation of protein samples by one-dimensional (1D) PAGE with LC/MS/MS. We developed a new label-free quantitative algorithm by combining measurements of spectral counting, ion intensity, and peak area on 1D PAGE-based proteomics. This algorithm has several improvements over other label-free quantitative algorithms: (i) Errors in p...

Label-free Quantitative Analysis of One-dimensional PAGE LC/MS/MS Proteome APPLICATION ON ANGIOTENSIN II-STIMULATED SMOOTH MUSCLE CELLS SECRETOME*□S

A widely used method for protein identification couples prefractionation of protein samples by one-dimensional (1D) PAGE with LC/MS/MS. We developed a new labelfree quantitative algorithm by combining measurements of spectral counting, ion intensity, and peak area on 1D PAGE-based proteomics. This algorithm has several improvements over other label-free quantitative algorithms: (i) Errors in pe...

Gel-Free Proteome Analysis Isotopic Labelling Vs. Label-Free Approaches for Quantitative Proteomics

iPRG-2015 Proteome Informatics Research Group Study: Differential Abundance Analysis in Label-free Quantitative Proteomics

The goal of the study is to detect the proteins that change in relative abundance among the four biological samples. Please note that this is not a study comparing database search engines or MS signal processing tools. Therefore, peptide identifications and their associated integrated peak intensities have been provided as starting points of the analyses. However, if you would like to start fro...

Label-Free Quantitative Analysis Using LC/MS